Dr. Eberhardt-Burkes collegiate career started in the Chemistry Department at Delaware State University in Dover, DE where she was awarded a scholarship to play womens basketball and run track. She graduated in 1995 and was awarded a National Science Foundation (NSF) Scholarship to finish her Masters and Ph.D through a joint program of Hampton University and Virginia Polytechnic Institute and State University (Virginia Tech) as a Doctoral Fellow. Her Masters thesis was entitled Micellar Properties of Spermicidal and Microbicidal Quaternary Ammonium Salts. During her doctoral program at Virginia Tech, she also joined the Clothing and Textiles department yielding a Doctoral degree in Clothing and Textiles with a cognate in Chemistry in April 2004. Her dissertation was entitled: Antibacterial and Laundering Properties of AMS and PHMB as Finishing Agents for Healthcare Workers Uniforms.
In the fall of 2005, Darlene (or Dr. E/E-B as her students affectionately calls her) joined North Carolina Central University (NCCU) as a Tenure-Track Assistant Professor in the Department of Human Sciences. While at NCCU, Dr. E-B has taught both undergraduate and graduate classes in the Textiles and Apparel Area while continuing her research in textile finishing for athletic uniforms, apparel entrepreneurship, African American dress and dress codes for students. In June 2012, Dr. E-B was awarded promotion and tenure at North Carolina Central University and the following week was asked to serve as Interim Chair of the Department of Human Sciences. She served as Interim for one year and is currently in her 3rd year serving as Chair and Director of Graduate Studies for the Department of Human Sciences. Even with her promotions, Dr. E-B continues her passion of teaching and advising students.